The ban applies from 8 pm 25 January until 12 pm 27 January.
Port Phillip Council is urging everyone intending to enjoy parks and beaches over these three days to play their part in keeping the City safe and clean.
"We had a hugely successful Christmas and New Year period with generally great behaviour along the foreshore and in our popular parks," Mayor Marcus Pearl said.
This promoted a fun family-friendly atmosphere as well as resulting in less waste needing to be picked up by Council cleaning crews.
"We want the last of the traditionally busiest days on our summer calendar to end on the same successful note, so we're asking everyone to again follow the alcohol and COVID-19 rules and to not litter."
Cr Pearl also urged St Kilda Botanical Gardens visitors to be mindful of these rules at the heritage-protected gardens which are home to endangered plants.
DJs or loud amplified music are also not allowed at the picnic-friendly Botanical Gardens.
Council's extensive summer management campaign, including extra public bins and beach cleans and regular patrols by local laws officers, is continuing until the end of March.
Victoria Police enforces the alcohol ban which carries a $200 fine. A glass ban always applies to beaches and foreshore reserves and parks.
The St Kilda Foreshore Alcohol ban (1 November-31 March), which also includes unopened containers, is already activated. Beaches and foreshore reserves (including Catani Gardens) from Langridge Street to Thackeray Street are included in that ban.
