Researchers at Karolinska Institutet have developed a molecular method able to detect whether or not bacteria respond to antibiotics within minutes. The findings are presented in the journal Nature Microbiology and the researchers now hope to develop a simple test for doctors to use.
Vicent Pelechano. Photo: Ryan Hull
"We are confident and hope that this can be one of many tools that doctors need to tackle antibiotic resistance, which is a serious and growing problem," says principal investigator Vicent Pelechano, associate professor at the Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet.
The method is called 5PSeq, is simple to use, and is based on sequencing the messenger RNA (mRNA) that the bacteria break down as they synthesise proteins. The measurements reveal how the bacteria are affected by different environmental factors, such as antibiotic treatments and other types of stress.
Rapid test for clinical use
The researchers tested the method on a total of 96 bacterial species from different phyla in complex clinical samples taken, for example, from faecal matter and the vagina, but also in compost samples. After only a matter of minutes, they were able to see whether or not the bacteria responded to antibiotic treatment; the effect was most salient after about half an hour.
3N Bio is a company that Dr Pelechano and some of his colleagues have started up in the KI Innovations incubator to develop the method and create a rapid molecular test for clinical use. They have now received financing from the Swedish Research Council to demonstrate proof of concept for such a test in collaboration with the Karolinska University Hospital.
"It's crucial that doctors can quickly find the right antibiotics for seriously ill patients with bacterial infections to reduce the unnecessary use of antibiotics," says Dr Pelechano. "Current methods of testing antibiotic resistance can take hours or even days, but often treatment needs to be given more promptly than that to avoid serious consequences for the patient. Because of this, a broad-spectrum antibiotic is often prescribed, which increases the risk of resistance."
Examine how bacteria interact
Apart from measuring antibiotic resistance, the method can be used to help researchers understand how bacteria handle stress and interact with each other and with their hosts. The researchers will continue to study complex gut samples to examine how the bacterial communities interact in our gut and contribute to health and disease.
The research was financed by the Ragnar Söderbergs foundation (Swedish Foundations' Starting Grant), the Swedish Research Council, the Knut and Alice Wallenberg Foundation (a Wallenberg Academy Fellowship), EU's Horizon 2020, among others. See the scientific article for a complete list. 3N Bio was co-founded by Vicent Pelechano and the two first authors of the paper, Susanne Huch and Lilit Nersisyan.
Publication
"Atlas of mRNA translation and decay for bacteria", Susanne Huch, Lilit Nersisyan, Maria Ropat, Donal Barrett, Mengjun Wu, Jing Wang, Valerie D Valeriano, Nelli Vardazaryan, Jaime Huerta-Cepas, Wu Wei, Juan Du, Lars M Steinmetz, Lars Engstrand, Vicent Pelechano. Nature Microbiology, online 22 May 2023, doi: 10.1038/s41564-023-01393-z.
