The dengue virus combines molecules from its RNA with mosquito saliva to foil the human immune system and spread dengue fever and related diseases. (Illustration by Emily Faith Morgan, University Communications)
The saliva of mosquitoes infected with dengue viruses contains a substance that thwarts the human immune system and makes it easier for people to become infected with the potentially deadly viruses, new research reveals.
Dengue has spread in recent years to Europe and the southern United States in addition to longstanding hotspots in tropical and subtropical areas such as Southeast Asia, Africa and Latin America. The new discovery, from a University of Virginia School of Medicine scientist and his collaborators, helps explain why the disease is so easily transmitted - and could eventually lead to new ways to prevent infection.
"It is remarkable how clever these viruses are. They subvert mosquito biology to tamp down our immune responses so that infection can take hold," said Dr. Mariano A. Garcia-Blanco, who recently joined the UVA faculty as chair of the Department of Microbiology, Immunology and Cancer Biology. "There is no doubt in my mind that a better understanding of the fundamental biology of transmission will eventually lead to effective transmission-blocking measures."
Dr. Mariano A. Garcia-Blanco, chair of the Department of Microbiology, Immunology and Cancer Biology, and colleagues found the connection. (UVA Health photo)
Garcia-Blanco suspects that researchers will find similar immune-dampening substances accompanying other mosquito-borne infections such as Zika, West Nile and yellow fever. "Our findings are almost certainly going to be applicable to infections with other flaviviruses," he said. "The specific molecules here are unlikely to apply to malaria, but the concept is generalizable to viral infections."
Understanding Dengue
Approximately half the world's population is at risk for dengue and roughly 400 million people are infected every year. Dengue's symptoms, including fever, nausea and skin rash, are often mistaken for other diseases. Most people will have mild cases, but about 1 in 20 will develop severe illness that can lead to shock, internal bleeding and death. Unfortunately, it's possible to contract dengue repeatedly, as it is caused by four related viruses transmitted primarily by the Aedes aegypti species of mosquito. There is no treatment, but the new discovery from Garcia-Blanco and his colleagues identifies an important contributor to the disease's spread as researchers seek to find better ways to combat it.
Garcia-Blanco and his team found that infected mosquitoes' saliva contained not just the expected dengue virus, but a powerful conspirator: molecules produced by the virus that can blunt the body's immune response. The injection of these molecules, called sfRNAs, during the mosquito bite makes it more likely that the victim will become infected with dengue, the scientists conclude.
"By introducing this RNA at the biting site, dengue-infected saliva prepares the terrain for an efficient infection and gives the virus an advantage in the first battle between it and our immune defenses," the researchers write in a new scientific paper outlining their findings.
Scientists who study mosquitoes previously had suspected that the insects' saliva might contain some type of payload to enhance the potential for infection. Garcia-Blanco's team's new findings pinpoints one weapon in the viruses' arsenal and opens the door to finding new ways to help reduce transmission and control the disease's spread. For now, the best way to avoid getting seriously sick with dengue remains to avoid getting bitten.
"It's incredible that the virus can hijack these molecules so that their co-delivery at the mosquito bite site gives it an advantage in establishing an infection," said researcher Tania Strilets, a graduate student with Garcia-Blanco and co-first author of the scientific paper. "These findings provide new perspectives on how we can counteract dengue virus infections from the very first bite of the mosquito."
Findings Published
The researchers published their findings in the scientific journal PLOS Pathogens. The team consisted of Shih-Chia Yeh, Strilets, Wei-Lian Tan, David Castillo, Hacène Medkour, Félix Rey-Cadilhac, Idalba M. Serrato-Pomar, Florian Rachenne, Avisha Chowdhury, Vanessa Chuo, Sasha R. Azar, Moirangthem Kiran Singh, Rodolphe Hamel, Dorothée Missé, R. Manjunatha Kini, Linda J. Kenney, Nikos Vasilakis, Marc A. Marti-Renom, Guy Nir, Julien Pompon and Garcia-Blanco. Most of Garcia-Blanco's work on the project was conducted while he was at Duke-NUS Medical School and the University of Texas Medical Branch.
The researchers reported no financial interest in the work.
Garcia-Blanco's research for the project was supported by the Duke-NUS Signature Research Programme in Emerging Infectious Diseases funded by the Agency for Science Technology and Research (A*Star Singapore) and by the National Institutes of Health's National Institute of Allergy and Infectious Diseases, grant AID P01 AI150585.
