Researchers from the IMBB and the Very High Field NMR Center in Lyon introduce a novel liquid crystal that is based on filamentous type 1 pili from E. coli, resists harsh experimental conditions and allows the measurement of residual dipolar couplings for proteins, nucleic acids and small molecules by NMR.
In NMR spectroscopy, residual dipolar couplings (RDCs) have emerged as one of the most exquisite probes of biological structure and dynamics. The measurement of RDCs relies on the partial alignment of the molecule of interest with respect to the external magnetic field, for example by using a liquid crystal as a solvent.
Researchers from the IMBB and the Very High Field NMR Center in Lyon now established E. coli type 1 pili as an alternative, liquid-crystalline alignment medium for the measurement of RDCs. To achieve alignment at pilus concentrations that allow for efficient NMR sample preparation, wild-type pili were elongated by recombinant overproduction of the main structural pilus subunit. Building on the extraordinary stability of type 1 pili against spontaneous dissociation and unfolding, the researchers show that the pilus-based medium is compatible with challenging experimental conditions such as high temperature, the presence of detergents, organic solvents or very acidic pH, setting it apart from most established alignment media. Using human ubiquitin, HIV-1 TAR RNA and camphor as spectroscopic probes, the applicability of the medium for the determination of RDCs of proteins, nucleic acids and small molecules is demonstrated.
The study shows that type 1 pili represent a very useful alternative to existing alignment media and can readily assist the characterization of molecular structure and dynamics by NMR spectroscopy.
Link to the paper in external page"Angewandte Chemie".
