Several hundred genes are associated with autism spectrum disorders. Some patients are only mildly affected, while others have severe disabilities. In addition to characteristic symptoms like difficulties in social interaction and communication with other people, as well as repetitive-stereotypic behaviors, patients with mutations of the gene CHD8 oftentimes have intellectual disabilities and macrocephaly – an unusually large brain. How CHD8 causes these symptoms has long been unclear.
Tiny artificial brains
Since CHD8 mutations affect the brain at a very early stage of its development, it has proven difficult for scientists to get the full picture. Over the past years, many researchers therefore used mice as model organisms to better understand what is going on. "But mice with a CHD8 mutation barely showed the symptoms human patients are showing. The effects in mice are not comparable to humans. We needed some kind of human model," Professor Gaia Novarino explains.
Together with collaborators from the Italian Human Technopole institute, the European Institute of Oncology, and the University of Milan, as well as the Allen Institute for Brain Science, USA, Novarino and her team at ISTA turned to organoids. These simplified miniature versions of organs are made from stem cells, which have the ability to become almost every other type of cell. By creating the right circumstances and giving the proper input at just the right time, the scientists were able to mimic developmental processes to create basic versions of brain tissue the size of lentils. "Organoids are the only way you can study human brain development at such an early phase," says Bárbara Oliveira, postdoc in the Novarino group and one of the authors of the study.
CHD8 mutations disrupt balance of neuron production
In petri dishes the team created brain organoids with and without mutations of the gene CHD8. "After some time, we could see by eye that the mutant organoids were much bigger. That was the first evidence that the model works," her colleague and co-author, PhD student Christoph Dotter, describes. Like patients with a CHD8 mutation, the organoids were showing signs of brain overgrowth.
Getting an overview of all the cell types in the organoids, the team notices something very early on: The mutant organoids started to produce a specific type of neurons, inhibitory neurons, much earlier than the control group. So called excitatory neurons, however, were produced later. Furthermore, the mutant organoids produced much more proliferating cells that later on produce a larger amount of this kind of neurons. Over all, the scientists concluded, this leads to them being significantly bigger than the organoids without CHD8 mutations correlating with patient's macrocephaly.
Starting to understand our brain
Like previous studies by the Novarino group, their recent study shows just how important time is when studying autism. "Looking at different time points gives us the information that what you see in the end might not be the full picture of how the brain of a patient developed – much more might have happened before," says Novarino. "We still have a limited understanding of how different trajectories affect functions of the brain." To one day help patients with a CHD8 mutation, the basics of brain development need to be better understood. By reproducing genetic and clinical features from ASD patients in brain organoids, the Novarino group was able to make an important contribution.
Publication:
Carlo E. Villa, Cristina Cheroni, Christoph Dotter et al. 2022. CHD8 haploinsufficiency links autism to transient alterations in excitatory and inhibitory trajectories. Cell Reports. DOI: 10.1016/j.celrep.2022.110615
Funding information:
The ISTA project part was supported by funding from the European Union's Horizon 2020 research and innovation program (ERC) and the Austrian Science Fund (FWF).
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