Monitoring cancer cells effectively can help physicians with treatment and management, thus reducing cancer-related mortality. Can non-invasive technologies pave the way for improved monitoring to reduce cancer mortality rates? Diagnostic platforms that non-invasively measure the electrical properties of cancer cells offer promise in the early detection of cancer drug resistance and metastasis. Research has shown that it is possible to understand a cancer type and its drug resistance status from cellular permittivity and conductivity data. In fact, there is an increasing demand for analytical methods that can rapidly measure a cell's electrical properties.
Electrorotation (ROT) offers one such route to capture cellular properties by inferring permittivity and conductivity from a cell's movement in an electric field. This allows the characterization of the cell type and state by profiling its frequency-dependent rotational movement under a modulated electric field. However, there are limitations. The challenge is that the capture, measurement, and replacement of cells is quite cumbersome and lowers the throughput of ROT platforms, where throughput refers to the number of cells that a given technology can analyze at any given time.
Recently, researchers from Tokyo University of Science (TUS) developed a continuous flow ROT (cROT) to address conventional ROT's drawbacks. The new platform leverages microfluidics to continuously measure cellular dynamics and simultaneously capture cells to collect measurements on one device. The group's validated findings were recently published in Lab on a Chip on 23 October 2023.
"I discovered that cancer cells had vastly different responses to electric fields while they looked similar. This implied a certain degree of individuality, and the idea of discerning the differences using ROT intrigued me," explains Dr. Masahiro Motosuke, a Professor in the Department of Mechanical Engineering at TUS and the project's Principal Investigator. He further adds, "However, gathering accurate data using ROT requires the precise placement and removal of a single cell, and I wanted to make the process of analyzing many cells easier."
The researchers fabricated the new device with redesigned interdigitating electrodes that induce cell rotation and a microchannel for cell passage. The electrode geometry increases the number of cells that can be analyzed and reduces the time required to replace a cell as measurements are collected. The electrical field applied within the microchannel enables analyzing rotational behavior from a continuous flow of cells. Together, these improvements increase the automated system's throughput. The research team validated the system's accuracy by obtaining cell membrane permittivity and cytoplasm conductivity measurements from HeLa cells, a human cell line commonly used in research.
"We significantly increased measurement throughput to 2,700 cells per hour with our cROT technique," says Prof. Motosuke about the report's most significant findings. "Furthermore, the device does not require precise cell manipulation and takes advantage of rapid image processing when processing the cells' electrical data," he adds further. Other advantages of the new system are its high degree of automation and ease of installation or removal.
The cROT device indeed demonstrates a remarkable enhancement in throughput when contrasted with traditional ROT platforms. While conventional ROT techniques typically process 10 to 20 cells per hour, the cROT system achieves an impressive throughput of 2700 cells per hour, which is more than 100 times higher. Furthermore, the cROT system significantly minimizes the time necessary for cell replacement.
Prof. Motosuke envisions a promising future for the cROT system that the team has developed. "With our cROT technique, we've unlocked the ability to delve into the subtle intricacies of single-cell dynamics, including aspects like cell physiology, the state of the cell membrane, and the concentration of intracellular ions," he emphasizes. He anticipates that the swift and accurate analyses offered by this cutting-edge approach will be a catalyst for substantial advancements in the realms of cancer drug development, diagnosis, and novel cell-based therapies. This breakthrough technology opens doors for collaboration and adoption by prominent players in the oncology industry, potentially revolutionizing the way we combat cancer.
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Reference
DOI: https://doi.org/10.1039/D3LC00301A
About The Tokyo University of Science
Tokyo University of Science (TUS) is a well-known and respected university, and the largest science-specialized private research university in Japan, with four campuses in central Tokyo and its suburbs and in Hokkaido. Established in 1881, the university has continually contributed to Japan's development in science through inculcating the love for science in researchers, technicians, and educators.
With a mission of "Creating science and technology for the harmonious development of nature, human beings, and society," TUS has undertaken a wide range of research from basic to applied science. TUS has embraced a multidisciplinary approach to research and has undertaken intensive study in some of today's most vital fields. TUS is a meritocracy where the best in science is recognized and nurtured. It is the only private university in Japan that has produced a Nobel Prize winner and the only private university in Asia to produce Nobel Prize winners within the natural sciences field.
Website: https://www.tus.ac.jp/en/mediarelations/
About Professor Masahiro Motosuke from Tokyo University of Science
Dr. Masahiro Motosuke is a Professor in the Department of Mechanical Engineering and the Director of the Motlab (Micro/Nanoscale Thermofluidics Research Group) at Tokyo University of Science, Japan. He earned his Ph.D. in 2006 from the School of Integrated Design Engineering at Keio University, Japan. Prof. Motosuke researches sensing and control of thermofluidic behaviors at the micro/nanoscale level and the development of novel micro/nanodevices to improve the quality of life of people and society. He has published over 150 peer-reviewed articles since 2002.
Funding information
A part of this research was supported by Grant-in-Aid for Scientific Research (B) No. 22H014190 from the Ministry of Education, Culture, Sports, Science, and Technology (MEXT), Japan. Some of the microfabrication was performed at The University of Tokyo, supported by "Advanced Research Infrastructure for Materials and Nanotechnology in Japan (ARIM)" of the Ministry of Education, Culture, Sports, Science and Technology (MEXT) proposal number JPMXP12yyxx1234.
