Emergency services have responded to a two-vehicle collision in Winchelsea overnight.
It's believed two vehicles collided on Winchelsea-Deans Marsh Road just after 10pm.
The female occupant of one of the vehicles has died at the scene, the other female occupant from that vehicle has been taken to hospital with serious but non-life-threatening injuries.
The male driver and only occupant of the second vehicle has been taken to hospital with non-life-threatening injuries.
The exact circumstances surrounding the collision are yet to be determined and it will be investigated by Major Collision Investigation Unit detectives.
Anyone who witnessed the collision, with dashcam footage or information is urged to contact Crime Stoppers on 1800 333 000 or www.crimestoppersvic.com.au.