The primary cilium is an appendage located on the surface of cells. Here you can see samples of human medulloblastoma representing SHH, WNT, Group 3 and Group 4. The medulloblastoma cells are pictured in blue. In SHH and WNT you can see primary cilia (green lines) that grow from the basal body (magenta dots) on their surface. These green structures are not present in Group 3 and Group 4. Yellow insets show high magnification images.
Present or absent, primary cilia still have an effect
The researchers evaluated over 100 cases of human medulloblastoma and found that primary cilia are abundant in the SHH and WNT groups, but rare in group 3 and group 4.
Han established the role of primary cilia in SHH medulloblastoma in previous research. Less is known about cilia's role in the other groups. Using mouse models of WNT and group 3 medulloblastoma, the scientists revealed primary cilia can either promote or suppress cancer depending on their underlying mechanisms of action.
"We found that in WNT tumors that have primary cilia, those cilia are aiding tumor development," Han said. "In group 3 tumors where the primary cilia are missing, those cilia act as a tumor suppressor, so their absence is what contributes to the cancer."
Mechanisms provide basis for new treatment approaches
In WNT medulloblastoma, primary cilia facilitate the translation of mRNA encoding β-catenin, which is part of a protein complex involved in signaling in the WNT pathway. Results from Han and his team suggest a molecule that targets the RNA binding proteins that regulate translation of β-catenin should be explored to treat WNT medulloblastoma.
In group 3 medulloblastoma, a lack of primary cilia disrupts cell cycle control and destabilizes the genome by abnormally activating CDK1. Previous research by St. Jude investigators supports the notion that targeting factors associated with the cell cycle could potentially treat medulloblastoma.
Group-specific therapies such as these would be useful because current patients with medulloblastoma generally receive the same treatments (surgery, chemotherapy and radiation). The findings also suggest that translation and cell cycle are mechanisms worth investigating in other diseases that involve primary cilia (ciliopathies).
"These findings have implications beyond cancer to other ciliopathies where there may also be a disruption of translation or the cell cycle. These latest results are worth investigating in these very diverse human diseases that affect people from their brains to their toes," Han said.
Authors and funding
The study's first author is Yong Ha Youn, formerly of St. Jude. Other authors are Shirui Hou and Chang-Chih Wu, formerly of St. Jude; Daisuke Kawauchi, National Center of Neurology and Psychiatry Tokyo; Makoto Taketo, Graduate School of Medicine Kyoto University; Richard Gilbertson, Department of Oncology and CRUK Cambridge Institute; and Brent Orr, Giles Robinson, David Finkelstein and Martine Roussel of St. Jude.
The study was supported by grants from the National Institutes of Health (CA021765), the Sontag Foundation Distinguished Scientist Award and ALSAC, the fundraising and awareness organization of St. Jude.
Read the full text of the article:
Primary cilia in medulloblastoma: mechanisms provide treatment opportunity.
Genes and Development 2022
St. Jude Children's Research Hospital
St. Jude Children's Research Hospital is leading the way the world understands, treats and cures childhood cancer and other life-threatening diseases. It is the only National Cancer Institute-designated Comprehensive Cancer Center devoted solely to children. Treatments developed at St. Jude have helped push the overall childhood cancer survival rate from 20% to 80% since the hospital opened more than 60 years ago. St. Jude freely shares the breakthroughs it makes, and every child saved at St. Jude means doctors and scientists worldwide can use that knowledge to save thousands more children. To learn more, visit stjude.org or follow St. Jude on social media at @stjuderesearch.
