Genetic analysis finds evidence suggesting that acoustic fat bodies in the heads of toothed whales were once the muscles and bone marrow of the jaw.
Illustration of the body plan of a toothed whale, with a cross section of the head showing the melon (dark yellow) and the extramandibular fat bodies (light yellow) which are key organs for using sound such as echolocation. (Hayate Takeuchi, Takashi Fritz Matsuishi, Takashi Hayakawa. Gene. January 20, 2024)
Dolphins and whales use sound to communicate, navigate and hunt. New research suggests that the collections of fatty tissue that enable toothed whales to do so may have evolved from their skull muscles and bone marrow.
Scientists at Hokkaido University determined DNA sequences of genes which were expressed in acoustic fat bodies-collections of fat around the head that toothed whales use for echolocation. They measured gene expression in the harbor porpoise (Phocoena phocoena) and Pacific white-sided dolphin (Lagenorhynchus obliquidens). Their findings were published in the journal Gene.
The evolution of acoustic fat bodies in the head-the melon in the whale forehead, extramandibular fat bodies (EMFB) alongside the jawbone, and intramandibular fat bodies (IMFB) within the jawbone-was essential for sound use such as echolocation. However, little is known about the genetic origins of those fatty tissues.
"Toothed whales have undergone significant degenerations and adaptations to their aquatic lifestyle," said Hayate Takeuchi, a PhD student at Hokkaido University's Hayakawa Lab and first author of the study. One adaptation was the partial loss of their sense of smell and taste, along with the gain of echolocation to enable them to navigate in the underwater environment.
The researchers found that genes which are normally associated with muscle function and development were active in the melon and EMFBs. There was also evidence of an evolutionary connection between the extramandibular fat and the masseter muscle, which in humans connects the lower jawbone to the cheekbones and is a key muscle involved in chewing.
The extramandibular fat bodies (EMFB) near the ear have a very high expression of the protein MYH16, a specialized protein typically found in the masseter muscle-indicating an evolutionary relationship between them. (Hayate Takeuchi, Takashi Fritz Matsuishi, Takashi Hayakawa. Gene. January 20, 2024)
"This study has revealed that the evolutionary tradeoff of masticatory muscles for the EMFB-between auditory and feeding ecology-was crucial in the aquatic adaptation of toothed whales," said Assistant Professor Takashi Hayakawa of the Faculty of Environmental Earth Science, who led the study. "It was part of the evolutionary shift away from chewing to simply swallowing food, which meant the chewing muscles were no longer needed."
Analysis of gene expression in the intramandibular fat detected activity of genes related to immune functions, such as the activation of some elements of the immune response and regulation of T cell formation.
The Stranding Network Hokkaido (SNH) is another important aspect of the research, as the samples used in this study were collected by them. SNH has collected specimens of stranded whales in the seashore and river mouth in Hokkaido. "Long-term communication with local people and communities in Hokkaido has enabled researchers to conduct various studies of whale biology, including our surprising findings," said Professor Takashi Fritz Matsuishi, the director of SNH.
(From left) Hayate Takeuchi, Takashi Fritz Matsuishi, and Takashi Hayakawa, authors of the study. (Photo: Hayate Takeuchi, Takashi Fritz Matsuishi, and Takashi Hayakawa)
Original Article:
Hayate Takeuchi, Takashi Fritz Matsuishi, Takashi Hayakawa. A tradeoff evolution between acoustic fat bodies and skull muscles in toothed whales. Gene. January 20, 2024.
DOI: 10.1016/j.gene.2024.148167
Funding:
This study was supported by Japan Society for the Promotion of Science (JSPS) KAKENHI (19K16241, 20H04987, 21H04919, 21KK0106), JSPS Bilateral Joint Research Project (JPJSBP 120219902), JSPS Core-to-Core Programme (JPJSCCA 20170005, Wildlife Research Centre of Kyoto University); Global Centre for Food, Land and Water Resources and Sousei Tokutei Research, Hokkaido University.
